Transcriptome analysis of developing castor bean seeds and identification of ricinoleic acid biosynthesis genes

Ricinoleic acid is a kind of unsaturated fatty acids in oil castor bean (Ricinus communis) seeds with wide application value. However, there little transcriptomic information on genes related to ricinoleic biosynthesis beans. To better understand the regulation mechanism biosynthesis, immature at three developmental stages (S1, S2, and S3 corresponding 15, 30, 45 d after pollination) were collected. The results indicated that accumulation increased gradually during seed development, reached maximum value late development (45 pollination). Furthermore, RNA sequencing was conducted analyze transcriptome developing stages. Totals 9 875 differentially expressed (DEGs) identified among time points. Based annotation information, 49 DEGs lipid screened all DEGs. Through cluster analysis DEGs, ten increasing FPKM values from S1 selected as candidate key enzymes, since they showed similar patterns increase development. data 10 enzyme further validated by RT-qPCR. Ultimately, putative model correlated built. Our study series revealed proposed molecular through transcriptional analysis. It broadens our knowledge also provides theoretical foundation for genetic engineering can improve production beans well other plants.